Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.)
Identifieur interne : 000192 ( France/Analysis ); précédent : 000191; suivant : 000193Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.)
Auteurs : G. Blanc [France] ; L. Lardet [France] ; A. Martin ; J. L. Jacob [France] ; M. P. Carron [France]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2002-06.
English descriptors
Abstract
Somatic embryogenesis in Hevea is stimulated when the embryogenesis induction medium contains maltose, rather than glucose, fructose, or sucrose, in equimolarity (Blanc et al., 1999). Kinetic analyses were carried out on various physiological and biochemical indicators over the 8 weeks that the induction phase then expression of somatic embryogenesis can take. Embryogenesis induction in the presence of glucose, fructose or sucrose revealed strong callus growth in the first 3–4 weeks, associated with a high intra‐ and extracellular hexose content, a high starch content and a substantial decline in protein synthesis. In the presence of maltose, callus growth was slow and only half that seen with sucrose. This morphogenetic behaviour is associated with a drop in endogenous hexose and starch contents, and an increase in protein synthesis in the first three weeks of culture. The induction of embryogenesis in the presence of maltose was uniform and twice as fast as with sucrose supply. At the end of culture, peroxidase activity, antioxidant and membrane protein contents increased in these calluses; these characteristics may be associated with somatic embryo organization and with the maintenance of effective membrane integrity within a nutrient environment that has become limiting. These new results tally with data in the literature on the roles of sugars, and provide some precise information with regard to the ‘carbohydrate deficit’ hypothesis usually put forward to explain maltose action. An analysis of these results led to the hypothesis that regulation of endogenous hexose contents at a low level, through slow maltose hydrolysis, was a key element of the biochemical signal leading this callus towards somatic embryogenesis.
Url:
DOI: 10.1093/jexbot/53.373.1453
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001818
- to stream Istex, to step Curation: 001817
- to stream Istex, to step Checkpoint: 000901
- to stream Main, to step Merge: 002D91
- to stream Main, to step Curation: 002C16
- to stream Main, to step Exploration: 002C16
- to stream France, to step Extraction: 000192
Links to Exploration step
ISTEX:45A20967275A25662C389FD16BB6B5CD48261BEALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.)</title><author><name sortKey="Blanc, G" sort="Blanc, G" uniqKey="Blanc G" first="G." last="Blanc">G. Blanc</name></author><author><name sortKey="Lardet, L" sort="Lardet, L" uniqKey="Lardet L" first="L." last="Lardet">L. Lardet</name></author><author><name sortKey="Martin, A" sort="Martin, A" uniqKey="Martin A" first="A." last="Martin">A. Martin</name></author><author><name sortKey="Jacob, J L" sort="Jacob, J L" uniqKey="Jacob J" first="J. L." last="Jacob">J. L. Jacob</name></author><author><name sortKey="Carron, M P" sort="Carron, M P" uniqKey="Carron M" first="M. P." last="Carron">M. P. Carron</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:45A20967275A25662C389FD16BB6B5CD48261BEA</idno><date when="2002" year="2002">2002</date><idno type="doi">10.1093/jexbot/53.373.1453</idno><idno type="url">https://api.istex.fr/document/45A20967275A25662C389FD16BB6B5CD48261BEA/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001818</idno><idno type="wicri:Area/Istex/Curation">001817</idno><idno type="wicri:Area/Istex/Checkpoint">000901</idno><idno type="wicri:doubleKey">0022-0957:2002:Blanc G:differential:carbohydrate:metabolism</idno><idno type="wicri:Area/Main/Merge">002D91</idno><idno type="wicri:Area/Main/Curation">002C16</idno><idno type="wicri:Area/Main/Exploration">002C16</idno><idno type="wicri:Area/France/Extraction">000192</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.)</title><author><name sortKey="Blanc, G" sort="Blanc, G" uniqKey="Blanc G" first="G." last="Blanc">G. Blanc</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CIRAD, TA 80/03, F‐34398 Montpellier Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Lardet, L" sort="Lardet, L" uniqKey="Lardet L" first="L." last="Lardet">L. Lardet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CIRAD, TA 80/03, F‐34398 Montpellier Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Martin, A" sort="Martin, A" uniqKey="Martin A" first="A." last="Martin">A. Martin</name><affiliation><wicri:noCountry code="subField">5</wicri:noCountry></affiliation></author><author><name sortKey="Jacob, J L" sort="Jacob, J L" uniqKey="Jacob J" first="J. L." last="Jacob">J. L. Jacob</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CIRAD, TA 80/03, F‐34398 Montpellier Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Carron, M P" sort="Carron, M P" uniqKey="Carron M" first="M. P." last="Carron">M. P. Carron</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CIRAD, TA 80/03, F‐34398 Montpellier Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><title level="j" type="abbrev">J. Exp. Bot.</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2002-06">2002-06</date><biblScope unit="volume">53</biblScope><biblScope unit="issue">373</biblScope><biblScope unit="page" from="1453">1453</biblScope><biblScope unit="page" to="1462">1462</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">45A20967275A25662C389FD16BB6B5CD48261BEA</idno><idno type="DOI">10.1093/jexbot/53.373.1453</idno><idno type="PII">1460-2431</idno><idno type="local">531453</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Callus metabolism</term><term>Hevea brasiliensis</term><term>carbohydrates</term><term>regeneration</term><term>somatic embryogenesis.</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Somatic embryogenesis in Hevea is stimulated when the embryogenesis induction medium contains maltose, rather than glucose, fructose, or sucrose, in equimolarity (Blanc et al., 1999). Kinetic analyses were carried out on various physiological and biochemical indicators over the 8 weeks that the induction phase then expression of somatic embryogenesis can take. Embryogenesis induction in the presence of glucose, fructose or sucrose revealed strong callus growth in the first 3–4 weeks, associated with a high intra‐ and extracellular hexose content, a high starch content and a substantial decline in protein synthesis. In the presence of maltose, callus growth was slow and only half that seen with sucrose. This morphogenetic behaviour is associated with a drop in endogenous hexose and starch contents, and an increase in protein synthesis in the first three weeks of culture. The induction of embryogenesis in the presence of maltose was uniform and twice as fast as with sucrose supply. At the end of culture, peroxidase activity, antioxidant and membrane protein contents increased in these calluses; these characteristics may be associated with somatic embryo organization and with the maintenance of effective membrane integrity within a nutrient environment that has become limiting. These new results tally with data in the literature on the roles of sugars, and provide some precise information with regard to the ‘carbohydrate deficit’ hypothesis usually put forward to explain maltose action. An analysis of these results led to the hypothesis that regulation of endogenous hexose contents at a low level, through slow maltose hydrolysis, was a key element of the biochemical signal leading this callus towards somatic embryogenesis.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><noCountry><name sortKey="Martin, A" sort="Martin, A" uniqKey="Martin A" first="A." last="Martin">A. Martin</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Blanc, G" sort="Blanc, G" uniqKey="Blanc G" first="G." last="Blanc">G. Blanc</name></region><name sortKey="Carron, M P" sort="Carron, M P" uniqKey="Carron M" first="M. P." last="Carron">M. P. Carron</name><name sortKey="Jacob, J L" sort="Jacob, J L" uniqKey="Jacob J" first="J. L." last="Jacob">J. L. Jacob</name><name sortKey="Lardet, L" sort="Lardet, L" uniqKey="Lardet L" first="L." last="Lardet">L. Lardet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000192 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000192 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:45A20967275A25662C389FD16BB6B5CD48261BEA
|texte= Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Müll. Arg.)
}}
| This area was generated with Dilib version V0.6.25. |  |